MolMic

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Sec pathway

Ribosome, TF, SecB, SecA, SecYEG, Lep cleavage

Defining characteristic of Tat signal sequence

AxA sequence at the end. Also it's longer than Sec SS

Energy requirement for Tat

No ATP --> probably PMF?

Two hypotheses why proteins are targeted to Tat system?

1. Chaperones used for folding in cytoplasm.
2. Fold too quickly by themselves in cytoplasm which renders them incompatible with Sec.